Dive transect on the Swedish west coast

During the summer, the BalticSeaWeed blog did al ot of fieldwork, both at Askö on the east coast and Tjärnö on the west coast.

Among other things, we performed an inventory of algae populations along two transects (laid out measuring tape) outside Tjärnö on the salty west coast.

The scuba diver swims from the beach with a tape measure that has been attached at the waterline down to the depth where no more algae grow. Depending on water clarity, this may vary from a few meters to more than 20 meters depth.

Once the algae end, the diver takes out her slate (the single most important tool for any marine biologist) and begins by noting the depth and how much of the tape measure that’s been rolled out. Subsequently, the diver notes down all the algal species she sees and appreciates how much of them there are, on a 7-point scale (1, 5, 10, 25, 50, 75 and 100%).

When the diver has recorded all of this about the starting point, she swims slowly along the transect (tape measure) and continues to note the depth, length and species when it becomes a visible difference in the species that dominates, in order to produce a map of different “algal belts”.

Each “belt” is also sampled, using frames and bags. The diver uses a fixed size frame, which can be loose or attached to a bag, of a size usually 20×20 or 50×50 cm, depending on how many species and how much algae it is.

The diver puts the frame on the bottom, picks the largest algae by hand and puts them into the bag and then use a scraper to get off all the algae that grows within the frame and whisk them into the bag. It’s harder than it looks to work under water when everything is floating around.

For you to get an idea of how it works, Joakim Hansen, who helped out as dive buddy this summer, shared what he was filming with the BalticSeaWeed blog. Here’s how it looks when you scrape a frame.

Why, then have we done this, except that it’s very nice to go for a dive?

On these two sites, these inventories have been conducted for several years. In ecology, it is very important to have measurements that extend over a long period of time in order to see if there is a genuine change in the environment, or if it is just normal variations between years.

So during the cold, dark months, we will pick up our bags with frozen algae out of the freezer (there were over 30 of them), thaw them, sort them into piles according to species, dry and weigh and record in the protocols, thus getting the number of grams dry weight of each species that grew in each frame. By comparing our data with previous protocols, we can then see if it has become more or less of any species, and if any new species have appeared or if any have disappeared over the years.

Autumn preparations at Askö Laboratory

Last Monday it was time to put the seaweed out in the sea for overwintering. After some different trials of overwintering indoors in the Experiment Hall at the Askö Laboratory, with extra lights and air pumps, we have found that it is still difficult to get good enough water circulation and movement for the seaweed to be happy. It becomes brittle and falls apart come spring. But tying plants on to net cages and placing them on the sea floor at some meters depth works just fine!
The weather was amazing. Calm and brilliant sun. But the water level was too high, so now they are places too shallow to remain in the bay all winter. We will have to come back later and move them to a deeper waters. That will be a dry suit -job.

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In this year’s seaweed plantation is material from Gotland that we have collected in order to try and solve the question of if there is a third ecotype of bladderwrack. We know from before that in the Baltic Sea we find both summer reproducing (end of May-June) and autumn reproducing (end July-October) bladderwrack. But now it seems that we have found a third type, that reproduces more or less constantly throughout the entire season (May-October).

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The Sea Environment Seminar of 2013, was held at Vår Gård located in Saltsjöbaden outside Stockholm. In the morning Lena went for a walk alongthe beach and found plenty of free-floating bladderwrack balls, both in the wrack wall on the beach and also floating in the shallow water, rolling around.

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According to litterature, these do not reproduce sexually, and we have never seen any reproductive tips on this form of bladderwrack. Naturally, she collected several wrackballs. Since Lena had forgotten to bring a plastic bag (wich is something a true marine biologist always should keep on her/him), she had to go back to the hotel reception and ask for one. Imagine their surprised looks. The day after, Lena went out to ASkö and tied the wrackballs to little ceramic tiles. What we are looking for is if these freee floating forms will become sexual in spring if they have a fixed up and down. Now they are placed in the sea for the winter, and all we can do is wait for the return of sunlight and warmth in spring, when we can dive down and check if there are any reproductive tips on them.

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Day 5 EMBS – The final sprint

It’s not fun to get the presentation slot first out on the morning after the conference dinner. But Katarina from Estonia got a good attendance and gave an interesting presentation on the zebra mussel (Dreissena polymorpha) and its impact on the ecosystem of the heavily eutrophicated Pärnu Bay in southern Estonia.

Maillie Gall from Australia told A story of two sea urchins, in which she compared the population genetics of the two sea urchin species Heliocidaris erythrogramma and Heliocidaris tuberculata to see how far their larvae spreads. She has primarily examined whether the duration of the planktonic larval stage plays a role for the spreading distance. It was very nice to rest those weary eyes on some beautiful pictures of sea urchins and Australian waters.

Jennifer Loxton held one of the conference’s coolest presentations, according to me. She showed how a bryozoan (phylum Bryozoa, it’s an animal) that came into English waters recently from Japan, reproduce like crazy. With movies and beautiful microscope images, we see that most of the currently known bryozoans form one egg bump per individual, where a larva is formed which then swims away and form a new colony. The Japanese moss animal produces up to five bumps, simultaneously! Unbelievable. The animal is red in color and thrives in cold water with high salinity, so we’ll probably not see it at the Swedish coast.

Final speaker of the conference was Paul Somerfield from Plymouth Marine Laboratory, renowned marine biologist and statistician, who talked about how to use statistics and not place too much weight on that which is odd or rare if one wants to describe an overall pattern. The presentation was entitled “Putting the species back into community analysis “. Funny, easy to understand and relevant!

Day 4 EMBS Galway

Niall McDonough from European Marine Board started the day. European Marine Board is a partnership of 35 national marine / oceanographic research institutes from 20 European countries. Recently, it has also opened up for a membership of a consortium of universities. The Board is working as a platform to develop a common understanding of what is needed and what should be prioritized within marine research in Europe, and communicate this to policy makers in the EU. There are lots of interesting publications that can be download for free on their website http://www.marineboard.eu
For those who want to learn more about the sea, they are also running a project called Ocean Literacy, wich we will look into more closely and get back to you on.

Brenda Walles from the Netherlands present us with more information about oysters, wich we like a lot, especially with garlic. She has further investigated the possibilities of using oyster beds as erosion protection (bioengineers) on exposed coasts. Today, they are used mostly as breakwaters, but they also have a greater impact on sediment bottoms around them. The most important factor for optimum protection has proven to be the length of the reef, not surprisingly, but the oysters also have more beneficial bioengineering effects that could be utilized better than we do today. And they are so very tasty…

Katrin Bohn from Southampton University has participated in a study of re-colonization of old boat docks in Liverpool that were restored in the early 1980’s. The study was begun several years ago by Professor Stephen J. Hawkins. The restoration wincluded removing large amounts of accumulated sediment in the docks and putting back the gates in order to control the water flow. The water clarity and the oxygen concentration in the dock improved significantly during the first six years, and now mussels, sponges and sea squirts have moved in.

Day 2 EMBS Galway

After a very pleasant evening with barbecue, beer and talk, most of the conference participants have managed to get out of bed and into the Bailey Allen Hall at the University for the day’s first session on “Mapping habitats and determine the ecological status”. As the title indicates, most of the presentations have a more bureaucratic character.

Opening speaker is Dr. David Connor, a marine biologist with over 30 years of experience in marine monitoring and surveying (including MESH, which maps the Baltic Sea), who is now on a loan to the EU for four years to work with the EU’s Water Framework Directive (WFD).

David shows many of the maps that they are now working on in order to get an overview of Europe’s seabeds. The maps have a resolution of 250x250m per pixel. Imagine that for a terrestrial map…However, it is much more difficult, and expensive, to make detailed maps underwater.
In addition to identifying the type of bottom substrate (sediment, rock, sand) they aim to include biological factors, energy exchanges and much more. The goal is that the European seabeds will be mapped down to 5000 meters depth, with relevant factors and good resolution.

Matt Frost followed with a talk about the benthic habitats and their classification in the EU’s Water Framework Directive, and whether there is such a thing as a sustainable habitat loss and if you can measure it to calculate when it is no longer sustainable. The basis for this work is the EU’s Habitats Directive. The data he shows includes more than one hundred researchers’ work over some years, published in the chapter “Habitat thresholds” in the report State of Seas from 2010.

Matt highlights the problems with getting hold of reliable data from all countries, and the difficulties in collecting good quality data at different depths and in different environments.
He concludes by saying that more research is needed before we can set quantitative targets for habitats, that we need to work out better methods for mapping, and that it is not possible to either set or verify quantitative habitat targets at present.

Dr. Xabier Guinda presents how they have managed to implement the EU’s Water Framework Directive for intertidal and shallow areas in Spain, France and Portugal, and the methods they have used to identify them. Primarily, they have used transects, which is the same as we do in Sweden.

Artem Isachenko presents how they have been able to identify mudflats with the presence of Arctica islandica (Ocean quahog) in the White Sea (where we from the BalticSeaWeed blog are eager to go for collecting some seaweed). To find relevant bottoms with the right type of sediment, they use side-scan sonar in Rugozerskaya strait, and received some very fine maps of bottom topography.

They even managed to interpret the sonar signal to determine the density of the large, very thick shelled Ocean quahog in the sediment, where it lives burrowed. Very convenient and comfortable!

They also checked the calculated numbers by taking pictures of the mudbottoms and count the number of Ocean quahog siphons sticking out of the mud (they are easy to recognize). The study shows that Ocean quahog can live in densities up to 400 individuals per square meter!

Henna Rinne, our Finnish colleague and friend, finish off the habitat mapping theme by presenting data from FINMARINET, which she has worked on for almost ten years. The Project is part of Natura 2000, where they have worked with habitats sandbanks (1110), reefs (1170) and small islands and islets (1620). The numbers are the code of the habitat within Natura 2000.

One of the biggest problems they have encountered when trying to map the coast of Finland is to get data. It exists, but is classified. If they get hold of the data, they are not allowed to publish it. Being from Sweden, we recognize the problem and sympathize. Today when every row boat has an echo sonar and a GPS with better resolution than many charts, one might feel it is no longer necessary to protect the depth data from Charlie (which certainly may find whatever data necessary for an invasion via Google Maps).

After a cup of tea and a stretch of the old legs, it’s time for one of the conference’s two female keynote speakers (a total of 9). Professor Maria Byrne did her bachelor here in Galway, but has since moved to Australia and work with echinoderms.
The presentation is about how the echinoderms entire life cycle, from egg to larva to juvenile and adult, is affected by changes in the aquatic environment. For a species to be able to survive in a changed environment, all stages of the life cycle must be able to be completed. Echinoderms builds up their body with calcium carbonate, so the major threats are ocean acidification (see yesterday’s talk) and increased water temperature.

Two species of sea urchins has clearly been affected. A cold-water species creeps slowly down towards Antarctica and have decreased along the Australian East Coast (Great Barrier Reef), while a temperate species have increased in number and extent as the water has become warmer.

Maria and her research group has investigated whether the response to the stress of increasing temperature and acidification are different, depending on whether the species is a polar water species or from temperate waters. It seems that the polar species are most sensitive to a decrease in pH, whereas species from temperate and tropical waters seem to have a greater ability to cope with acidification. Lowered pH seems to be the most important factor for the survival and growth of sea urchin larvae.

If one then adds a temperature rise of 3 degrees Celsius to different acidification stages and try it on the tropical sea urchin Tripneustes gratilla, commonly farmed as food in Asia, one sees that there is a clear synergistic effect on the growth of the larvae, and in later stages of life also in the gonads, which is what you eat. Thus, if it just gets a bit warmer, but not much more acidic, the urchins from tropical waters thrive down in South Australia in the future. The question is how it will affect the rest of the ecosystem. Sea urchins are known to graze heavily on seaweed!

In Starfish, they have instead seen that a rise in temperature inhibit growth. It seems that the sensitivity lies in the planktonic stage, so that species that have larvae with short planktonic stage, who does not need to build skeletons before they settle on the bottom, are less affected.

It’s always great fun to listen to someone who has worked for long in one area and it is a shame that time passes so quickly.

It’s….the 48th European Marine Biology Symposium!

This year’s edition of the EMBS, the 48th, will be held in the charming city of Galway on Ireland’s west coast.
During the week, you, dear BalticSeaWeed blog reader,will be able to keep up with the events, which we do our best to serve piping hot.

The programme is full of interesting presentations and posters. New for this year is the possibility to also present posters with a video on YouTube in order to really reach out with the results. We think that’s a brilliant idea, since Poster sessions are often a bit crowded and bustling, where long explanations and discussions can be difficult.
We also note that both Finnish and Estonian colleagues will contribute with interesting seaweed-talk.

Naturally, we hope to take a stroll along the beach to collect some seaweed for the herbarium. We have already found the seaweed products in stores.

The big question is which team will take home the Yellow Submarine this year. Don’t miss out on this!

Field season in full bloom

The days are just soaring by as the field season hits its high peak.

Helping us in this organized chaos is Frances Ratcliffe, volonteering marine biology student from UK. It’s such a help to have an extra pair of hands. We are very glad to have her with us.

Frances and bladderwrack!

Frances and bladderwrack!

The week before last was spent at the Askö laboratory, were we ran the Baltic Sea part of a two-station experiment wich will look closer on the effects of grazing snails on seaweed.

First, we put the seaweed (Fucus vesiculosus and Fucus radicans) grow a while without any grazers, in order to get undamaged tissue.

Seaweed growing

Seaweed growing

After a couple of weeks, we placed the tips in jars with gastropods (marine snails) and let them graze for a couple of days. We so hope that they were hugry and ate a lot, so that we can see the grazing damage under the microscope later on.

45 jars with gastropods and an even flow of water. Amazing what you can build with a pipe and some hose.

45 jars with gastropods and an even flow of water. Amazing what you can build with a pipe and some hose.

Once the seaweed was grazed, we collected some Ulva intestinalis, a green algae, and made it release its spores.

<img src="http://tangbloggen.files.wordpress.com/2013/07/entero1.jpg?w=300" alt="Ulva intestinalis spores makes the water go green.” width=”300″ height=”224″ class=”size-medium wp-image-910″ /> Ulva intestinalis spores makes the water go green.

What we hope to see is if the spores will be able to grow on the grazed surface of the seaweed.

<img src="http://tangbloggen.files.wordpress.com/2013/07/sdd201.jpg?w=300" alt="We have seeded the grazed seaweed by poruing spores of Ulva intestinalis in the water” width=”300″ height=”224″ class=”size-medium wp-image-920″ /> We have seeded the grazed seaweed by poruing spores of Ulva intestinalis in the water.

Let’s keep our fingers crossed and hope that it grows!

The week that was and this week, is spent at Tjärnö laboratory on the other side of Sweden, were we are running the same experiment, but in saltier water and with Ascophyllum nodosum instead of Fucus radicans and with other gastropods.

Our lives have been made so much easier thanks to Marit, who is doing her master thesis together with us. She has tended to our experiment so that we didn’t have to go back and forth between the coasts like crazy. Thank you Marit!
Meanwhile, she has also done an enormous fieldwork on a very interesting study on gastropods and seaweed, that we hope to write more about here on the BalticSeaWeedBlog.

Marit shows her disected gastropods.

Marit shows her disected gastropods.

it is always nice to meet and talk about seaweed. We had many nice discussions on this fascinating topic.

Frances, Marit and Lena discuss seaweed at Tjärnö Laboratory.

Frances, Marit and Lena discuss seaweed at Tjärnö Laboratory.

Seaweed course in Denmark

The week has been spent in Denmark, on a full Sunday to Sunday course to learn more about macroalgae.
With a schedule so full you can hardly fit it to a paper, the hopes and expectations to become a real seaweed guru are high.
There are 11 PhD students partaking, all from different countries!
The course is arranged by Aarhus University, as you can see in their newsletter (sorry, only in Danish).

Our first day of the course, we went out to collect macroalgae in the field. We started at the pier next to Ebeltoft harbour, where the sun shone and it was lee on the inside where we were working. I took this years “first swim without a drysuit in Nordic waters”. It was a refreshing 16 degrees Celsius and I was in the water for about 10 minutes, collecting material. Here there were four Fucus species growing: Fucus spiralis, Fucus vesiculosus, Fucus evanescens and Fucus serratus. Jackpot!

Lots of seaweed!

Lots of seaweed!

After a field lunch of sandwiches, we headed north, to Grenaa. Here, the Marine Home Guard and their boat took us out on Kattegat! What a luxury! This is a new collaboration between the Home Guard and Aarhus University, so the Danish TV was out and filmed us. Click here and fast forward to 1:19 into the clip and there we are.

Monday evening and Tuesday was spent going through the collected material to determine the different species and look at their different physiological structures.
On Wednesday, we tried to reproduce sugar kelp ( Saccharina latissima ) by getting them to release spores. It didn’t quite go as planned, so we took some good old bladderwrack and got it to release eggs and sperm instead.

On Thursday, we went to Algcenter Grenaa and had a look around. Pictures from our visit you can see the Algcenter website

Anette Bruhn show us how they cultivate sugarkelp at the Algcenter

Anette Bruhn show us how they cultivate sugarkelp at the Algcenter

We also got to visit the Kattegat Center, located just across the parking lot. This is one of Denmark’s many fine aquariums that have long been on my wish-to-see list. It was just as good, if not better, as I had expected. There was even an exhibition of algae and their importance. It is totally worth a visit!

Friday and Saturday were devoted to compiling and analyzing all of our data, and then present it in four groups according to various themes (Taxonomy, Monitoring, Light and Cultivation).

Danish seaweed; red, brown and grøn

Danish seaweed; red, brown and grøn

International Seaweed Symposium – Day 4

With rested brains, it is once more time to stock up on more seaweed, both mentally and physically.

All things sweet and seaweedy

All things sweet and seaweedy

After an opening plenary lecture by Iain Neish about the importance of having a vision and being stubborn if we are to succeed with aquaculture, it was time for a cup of coffee, a slice of fruit cake and the day’s first mini-symposium.

Plenary with Iain Neish

Plenary lecture with Iain Neish


Mini Symposium: Cultivation of tropical red seaweeds

The most common species of red seaweed that are farmed are Eucheuma spp., Kappaphycus spp., Porphyra spp. and Gracillaria spp.
In Chile and Peru, it is primarily Gracillaria spp. that is farmed. In Chile, they seek to develop new methods to cultivate seaweed in the lab, instead of taking material from wild populations as many do today. They have also investigated whether it is possible to grow other commercial species.

In Malaysia, the Philippines and Indonesia, most seaweed is farmed using the fixed off-bottom technique in shallow waters. The trick is to place them deep enough so that the algae are not harmed by the intense sun during low tide.

Most algae in shallow waters are farmed using the off-bottom method.

Most algae in shallow waters are farmed using the off-bottom method.

In deeper water, they use the free-swing method, where only one end is fixed at the bottom. The downside is that it takes up quite a lot of space, and then they must be set at such a distance that they do not become entangled in each other.

The free swing method, attached at only one point.

The free swing method, attached at only one point.

Other methods for deeper water is something called single longline rafts, spider web rafts or floating triangle, depending on how you have designed the ropes. But these rafts are secured at all four corners and thus are more stationary. It also means that you can place them closer together, without risking entanglement.

In deeper waters, single longline rafts are common.

In deeper waters, single longline rafts are common.

Some growers use hanging baskets that the seaweed is floating freely in, which does not seem like a good idea to me. But this is still at the development stage. They use high pressure water hoses to remove unwanted growth of other seaweed (epiphytes).

Dr. Flower Msuya from Tanzania showed a summary of how seaweed cultivation has started and continued for the East African coast, with examples from Mauritius, Madagascar, Tanzania and Zanzibar course. The main problem to cope with is that they are now beginning to get problems with various diseases. There is much further research to do and a lot of mistakes to learn from. At the same time, a mini-symposium was held in the hall next to this, with the topic being diseases and parasites on seaweed. It’s a hot topic for the seaweed industry.

Presentations: Integrated aquaculture and introductions
In Australia, much yellowtail kingfish and tuna are farmed. At present, there is no cultured seaweed in Australia, so the researchers are now trying to find species suitable for cultivation along with fish farms in order to reduce emissions (IMTA, see previous posts). The species they are looking for are those that are good at taking up nitrogen from fish farms, but there should also be a market for the seaweed.

Kathryn Wiltshire from the University of Adelaide tested several species of red and brown seaweed to see which was best at taking up nitrogen and which grew fastest, in order to select species suitable for further experiments with the conditions that give the best performance.

Tom Schils from the University of Guam (you get extra points if you know where it is without looking it up) told us that coral reefs in Micronesia and the Pacific have very distinct algal communities, which are now threatened by introducing new varieties of these species bred for cultivation. A well-known example is the red alga Acanthophra spicifera that has taken over shallow waters on coral reefs around Hawaii.

Micronesia has a Biosecurity Plan, which seeks to identify and prevent threats to the marine environment, such as how to manage ballast water which is a great disseminator of species from one place to another.

Dr. Yang from China showed how the farming of the red alga Gracillaria spp. is along China’s 18,000-kilometer coastline and how China is now working to develop the use of integrated aquaculture. Between 1967-1980, 50-60% of China’s aquaculture consisted of cultured seaweed, mainly brown alga Saccharina japonica. Since then, the proportion of farmed fish, shrimp, crabs and clams increased. It leads to increased nitrogen load, and you need to cultivate more seaweed to not have problems with eutrophication.
The production of Gracillaria spp. is rising steadily, from 0.13 hectares in 2000 to 1,067 hectares in 2007. In 2011, the total cultivation area of Gracillaria was an astonishing 1,500 hectares!

Join us diving for seaweed

On Thursday, you can join us under the surface and experience what it is like to scuba dive WITHOUT GETTING WET OR COLD!

How on Earth will that be possible?

On Thursday 21st we will have a sneak start on World Water Day! Come to Aquaria and join the BalticSeaWeed blog, who will get into the water to check on a seaweed experiment. You can also help feeding the animals at Aquaria and get answers to everything you’ve always wanted to know about fish, and much, much more.

The program (in Swedish) is HERE, and the press release from Stockholm Universitety (also in Swedish) is HERE.

See you Thursday!

EllenS